Journal: Clinical Science (London, England : 1979)

Article Title: Caspase-1 inhibition mitigates neonatal hyperoxia-induced vascular and cardiopulmonary inflammation in neonatal rats

doi: 10.1042/CS20242275

Figure Lengend Snippet: Treatment with caspase-1 inhibitor decreased gene expression of ( A ) IL-1β, ( B ) GSDMD and protein expression of ( C ) IL-1β, ( D ) GSDMD in hyperoxia-exposed endothelial cells. ( E ) Representative western blots of IL-1β and of GSDMD in the endothelial cells. In addition, caspase-1 inhibitor also reduced gene expression of ( F ) IL-1β ( G ) GSDMD and protein expression of ( H ) IL-1β, ( I ) GSDMD in hyperoxia-exposed smooth muscle cells. ( J ) Representative western blots of IL-1β and of GSDMD in the smooth muscle cells. n = 4–5/group, data are mean ± SD, two-way ANOVA with Tukey’s multiple comparisons test. * P <0.05 ** P <0.01, *** P <0.001; **** P <0.0001. HYP, hyperoxia; PL, placebo; RA, room air; VX-765, caspase-1 Inhibitor.

Article Snippet: Human Umbilical Artery Endothelial Cells, HUAECs (Cat# 202–05n, Cell Applications, San Diego, CA) were cultured in a human MesoEndo growth medium (Cat# 212–500, Cell Applications, San Diego, CA).

Techniques: Gene Expression, Expressing, Western Blot

Journal: PLOS ONE

Article Title: Nutraceutical COMP-4 confers protection against endothelial dysfunction through the eNOS/iNOS-NO-cGMP pathway

doi: 10.1371/journal.pone.0316798

Figure Lengend Snippet: Proposed pathway for the effects of COMP-4 in the human arterial endothelial cell.

Article Snippet: Frozen human umbilical arterial endothelial cells (HUAEC, cat# 202-05n, Cell Applications, Inc., San Diego, CA) were thawed in a 37°C water bath.

Techniques: